Highlights:

Skills:

Protein Research Associate 07/2012 - 01/2013
Regeneron Pharmaceuticals, Tarrytown, NY United States
Industry: Biopharmaceutical
High Throughput purification of therapeutic proteins using Protein A/G Affinity chromatography, Perform Dialysis, Characterization through Western blot, SDS-PAGE, Caliper instrument.
Purification of antibodies (human and mouse subtypes)with Emerald High throughput purification system purifying approx. 24 samples in one run. Used protein A/G affinity chromatography. Also performed conjugation of purified antibodies with chemical dyes (Alexa A488) and phycobilliproteins.--
Research Associate 10/2011 - 07/2012
Technovax Inc., Tarrytown, NY United States
Industry: Biotechnology
Transfect CHO cells with self designed recombinant vectors, harvest protein, down stream processing and recovery of protein, determine concentration. Also generate stable cell line.
Established a continuous stable CHO cell line expressing HA therapeutic protein, its recovery & purification Performed stable/transient transfection in mammalian CHO cell line with Recombinant gene- vector construct,facilitating expression and secretion of Hemaglutinin (HA) protein extra-cellularly. Created monoclonal stable CHO cell line producing therapeutic protein and its scale up in spinners and small scale Bioreactor. ptimized processes involved in concentration and downstream processing of HA protein (FPLC, TFF etc.) and developed protocol for purification & recovery of the HA protein.--

St. Johns University 10/2009 - 12/2011
Queens, NY, United States
Degree: Master's Degree
Major:Biological and pharmaceutical Biotechnology
The degree entailed study on various branches of Biotechnology like analytical, Biochemistry, Gene technology, cell and toxicology, Bio-macromolecules etc.

Resume_ Ankita Dhar