Highlights:• Design Primers • Electrophoreses-PCR-DNA extraction- Large DNA Extraction- sequence analysis • Cloning process simulation • Analysis of metagenome data • Genome Mining –Whole genome analysis • Bacterial Polyphasic taxonomies: Polar lipid analysis-Amino acid analysis - Quinone analysis-Polyamine analysis - Carbohydrate analysis-Fatty acid analysis- Molecular Taxonomy • AntiSMASH – Clustal X- CLC Main Workbench • Bacterial Bioactivity analysis - secondary metabolites • Antibiotics-Microbial Assays: The cylinder-plate method and the turbidimetric assay • Antimicrobial effectiveness testing: Challenge test • Investigation of the protective substances in the formulation of drugs • Process validation • Process deviation • Development of serial dilution methods "subculturing of microbes and bacterial cultures" • Biochemical test for microbe screening • GMP in the pharmaceutical industry (certificates are available) • Clean room • Creation of the comprehensive file as Site master file (SMF) • Standard operating procedures (SOPs) • Various microbiological tests/identification of microbiological species • Sampling • Electrophoresis-PCR-DNA Extraction-Large DNA Extraction-Genome Sequence and Partial Sequence Analysis • Simulation of the cloning process • Analysis of metagenome data • Bacterial polyphasic taxonomies: polar lipid analysis - amino acid analysis - quinone analysis - polyamine analysis - carbohydrate analysis - fatty acid analysis - molecular taxonomy • Analysis and detection of new species and new genera in bacteria • AntiSMASH - Clustal X- CLC main workbench • Analysis of bacterial bioactivity - secondary metabolites • Cytotoxicity tests • MIC - minimum inhibitory concentration • DNP dictionary of natural products • LC-MS / MS analysis • HPLC analysis - fractionation • Isolation and purification of bioactive compounds • Analytical HPLC • Preparative HPLC • NMR preparation and initial assessment • Gliding bacteria - marine bacteria - myxobacteria - actinobacteria • Secondary metabolite co-cultivation • Flow-cytometry assay with FLOWJO software • Cell Biology • ELISA analysis • MTT assay • Western blot analysis • Molecular Docking for drug discovery • Biotechnology

Skills:Microbiologist

Goal:I have expertise in Pharmaceutical industries. I have studied and worked in Germany, and Iran . -I am an experienced Microbiologist and Molecular Biologist with a strong research background in genome analysis of bacteria and drug discovery. - I am interested in the development of biosynthetic gene clusters analysis and the discovery of new drugs for the treatment of antibiotic-resistant pathogens. - I am interested in Pharmaceutical industries,GMP,GLP , Quality assurance and etc thatswhy i have gotten certificates such as Biotechnology quality manager, and Industrial Pharma Management beside of vaccine design.

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