Highlights:

Skills:cytogenetics, FISH, Biomedical Genetics research, Microarray, Molecular genetics

Clinical Laboratory Cytogenetics Technician 05/2012 - current
Hamad Medical Corporation, Doha, Qatar
Industry: Healthcare
Master in Biomedical Genetics with 6+ years in Cytogenetics and FISH,from ?Patient’s Bone marrow/Blood Sample culture, Harvesting, Processing.G-Banding, C-Banding, NOR-Banding, Q-Banding.Giemsa-Wright Banding,karyotype and reporting.currently working at Hamad Medical Corporation,Doha.
?DNA Extraction. ?RNA Isolation-cDNA Synthesis. ?RT-PCR, Multiplex PCR. ?Maxwell 16 System Promega. ?STR-Analysis. ?Genetic Analyzer-STR. ?Agarose Gel Electrophoresis. ?Gen Amp PCR System. ?Sample collection documentation. ?FISH Technique. ?FISH Analysis and scoring. ?FISH Probes cut off and Lot validation. ?FISH troubleshooting and Reagents Validation. ?Microarray. ?Sub-culturing and Cryopreservation. ?Peripheral Blood Karyotype and Screening. ?Prepare slides for microscopic examination and Performs microscopic analysis for cytogenetic studies on biological specimens from cell cultures. ??Assist the supervisor in validating the reagents as well as FISH probes ?Assist the supervisor in monitoring the QC parameters for different instruments. ?CAP PT Testing, Laboratory and Procedural QC Compliance and execution.--
Laboratory technologist 06/2010 - 04/2010
ARTEMIS MEDICAL CENTER, India, Delhi, United States
Industry: Healthcare
Molecular techniques performing Primer design, RNA Isolation, DNA extraction, RT-PCR, light cycler, cDNA Synthesis, Gel electrophoresis, Southern Blotting, Western Blotting, Protein Assay comparative sensitivity, Patient Data recording, Cytogenetic slide preparation and analysis, FISH technique, Banding techniques, nuclear transfection, Cryopreservation, GFP –Fluorescence Imaging and Quantification, Media and Reagents preparation, Complete pro forma reports, Spectrophotometry.
Laboratory Material and Technical Set up, Implementing techniques and laboratory safety, laboratory assisting guidelines, developing and writing trial SOP. Patient Sample collection, Patient Informed consent data, Patients data recording, Patients sample culture, passage and processing, Molecular techniques performing Primer design, RNA Isolation, DNA extraction, RT-PCR, light cycler, cDNA Synthesis, Gel electrophoresis, Southern Blotting, Western Blotting, Protein Assay comparative sensitivity, Patient Data recording, Cytogenetic slide preparation and analysis, FISH technique, Banding techniques, nuclear transfection, Cryopreservation, GFP –Fluorescence Imaging and Quantification, Media and Reagents preparation, Complete pro forma reports, Spectrophotometry, Autoclave safety, CO2 Incubator maintenance and monitoring, Li-Cor Odyssey western blot imaging, Olympus fluorescence microscope, operates various scientific apparatus; assists with research projects and may perform independent research or performs various clerical and editorial duties; compiles, processes and analyzes data; performs supervisory and other training duties. Designing data collection forms, Coordinating with the ethics committee and standardizing the safety precautions. Archiving study documentation and correspondence; Preparing final reports and occasionally manuscripts for publication. Study and Analyze quantitative and qualitative assignments. Coordinate Research documents and plan, standardize the protocols and initiate. Perform and follow up the research design. Collect and analyze the report study and interpret the test results.--

University of Madras 04/2005 - 05/2008
Madras, Tamil Nadu, India
Degree: Bachelor's Degree
Major:Biotechnology
Minor:Molecular Developmental Biology
Biotechnology with 6 semester

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