Highlights:

Skills:Confocal microscopy, Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), Histology, Cryostat sectioning, 3D Analysis Software for Life Sciences: Volocity, Amira, Avizo, Image processing: Photoshop, ImageJ, Data analysis: Graphpad, Origin, Excel, SAS, Computer programming: Matlab, C, Mechanical loading, Fluorescent dye, protein&dye conjugation, Surgery, Cell culture, polymerase chain reaction (PCR), In vivo loading, Live cell imaging, Research, Microsoft office: Excel, Word, PowerPoint

Teaching Assistant 09/2010 - 06/2011
University of Delaware, Newark, Delaware United States
Industry: Mechanical Engineering
Teaching assistant in dynamics and heat transfer: grade homework and exams, attend discussion class and answer questions for students in office hours
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Research Assistant 09/2010 - 06/2015
University of Delaware, Newark, Delaware United States
Industry: Biomedical Engineering, Mechanical Engineering
Independently accomplished three research projects, from which established three innovative research protocols, published five papers, and presented in three core international conferences. Effectively partnering with internal and external collaborators and tutored junior lab members.
Projects: 1). Investigation of osteocyte cyto-architecture through bio-imaging approach o Histology: fixation, staining, plastic embedding, sectioning, polishing, mounting o 3D Confocal imaging of osteocyte and reconstruction using Volocity, Amira and software o TEM sample preparation and TEM imaging o Statistical analysis among various murine genotype and disease type using Graphpad, Origin, SAS software 2). Bone-muscle crosstalk: imaging and quantifying solute transport across periosteum o Refined surgery and dissection skill on mice o Confocal multi-track time-series x-z scanning to track the penetration of tracer inside the periosteum vs time o Image processing using ImageJ, Matlab, Adobe Photoshop software 3). Investigation of osteocyte pericellular matrix using a novel dual-colored fluorescence recovery after photobleaching (FRAP) approach o Matlab modeling to simulate the experiment: solute transport in the matrix o Fluid shear force and drag force quantification o Fluorescent dye conjugation with protein o Confocal imaging synchronized with mechanical loading:Confocal multi-channel multi-time imaging & Bose mechanical testing system o Image processing: Matlab programming 4). Mesenchymal stem cells isolation and cell culture 5). On-going project: ex-vivo intracellular calcium signaling--
Research Assistant 08/2009 - 07/2010
University of Science and Technology Beijing, Beijing, Beijing China
Industry: Metallurgical Engineering
Independently accomplished the project: separation of inclusions from steels through an electrolysis approach.
o Established a comprehensive experimental procedure, validated through parallel experiments: sample processing, electrolyte preparing, electrolyzing, ultrasonic vibration, vacuum filtration and classification-screening o Investigated the components of inclusions using scanning electron microscope X-ray energy dispersive microanalysis(SEM-EDX)--

University of Science and Technology Beijing 08/2006 - 07/2010
Beijing, Beijing, China
Degree: Bachelor's Degree
Major:Metallurgical Engineering
GPA: 3.6

Xiaohan Lai_Resume