Highlights:dual master degree holder with 12 years of research experience in Stem cell, Cancer, Neurodegenerative research. Skilled in Molecular Biology, Proteomics, Genomics, Biophysics, Immunohistochemistry & Advanced Microscopy domains. Broad experience in Lab management, scientific operations & Life science business development.

Skills:molecular biology, stem cells, cancer research, eye research, biochemistry

Goal:scientific research in biomedical and biomolecular sciences, life science business management and life science business analyst

Certification:research and laboratory management, procurement, Biochemical, chemical and fire safety certifications, trained in Risk assessment and Waste management from National University of Singapore and Singhealth from Singapore. Conflicts of Interest (COI) and Responsible Conduct of Research (RCR) certifications from Collaborative Institutional Training Initiative (CITI) at the University of Miami, USA. Class N3 laser license certification from National Environment Agency, Singapore. RCULA (Responsible Care & Use of Laboratory Animals) certification from SingHealth, Singapore.

Honor:Science Foundation Ireland fellowship in 2008-2010 from University College Dublin, Ireland. Scientific Outreach Officer in ‘Science Foundation Ireland Outreach program’ in Dublin, Ireland. Rotary International Scholarship award in 2006-2007 from Umea University, Sweden.

Research Associate 04/16/2015 - current
Singapore Eye Research Institute, Singapore, Singapore Singapore
Industry: Healthcare (medical research)
Working as a Research Associate in Singapore Eye Research Institute on various Research Projects
Project 1: Investigation of LGR5 as a potential retinal stem cell marker To characterize the expression of LGR5 in the adult mammalian retina with the intention of understanding the potential of LGR5 to aid in retinal stem cell isolation for therapeutic purposes. Responsibilities 1. To characterize expression of LGR5 in the adult mammalian retina • Identification of specific retinal cell type expressing LGR5. Analysis of anatomical distribution of LGR5 positive cells in the retina (identification of novel retinal stem cell niches). 2. To investigate the plasticity and regenerative capacity of LGR5 positive cells in the retina • Analyze proliferation, self-renewal, clonal expansion and response to injury in vitro and in vivo. • Assess ability to differentiate into retinal neuronal precursors under appropriate culture conditions. Project 2: Dental Pulp Stem Cells (DPSC) as potential alternative source of retinal stem cells Responsibilities 1. Isolation and establishment of human dental pulp stem cell cultures from extracted teeth 2. Differentiation of DPSC and Gene expression analysis of differentiated DPSC 3. Analysis of retinal neuronal functionality of differentiated DPSC in vitro Project 3: Differentiation of Primary Mouse Muller Glial towards Retinal Progenitor fate In Vitro Responsibilities 1. Establishment of primary muller cell cultures from mouse retinae and human retinae 2. Evaluation of expression of ASCL1 in primary mouse Muller cells qRT PCR, IHC and Western blotting.--
RA 02/10/2014 - 04/15/2015
Cancer Science Institute, Singapore, Singapore Singapore
Industry: Healthcare
Worked as a Researcher on identifying the causes of DNA methylation aberrations in tumor cells.
1. To delineate the functional aspects of P53 in DNA methylation & consequences of P53 mutation in cancer. 2. To identify genes and pathways that are regulated by TP53-DNMT1 complex and rescue the epigenetic role of TP53 in TP53 altered cells. Responsibilities •Identifying hypometylated CpGs linked to p53-mutations that correlate with gene repression. •Characterize p53-DNMT1 target genes by identifying p53 & AP1 binding sites in p53 defficient and Isogenic cell lines using CHIP. •Preparation of Libraries for CHIPseq (High-throughput sequencing - MiSeq). •Characterization of methylated cytocines on the promoters using bisulfite sequencing. •Determining methylation status of AP1 binding sites using Illumina infinium 450k platform.--
Scientist 02/12/2011 - 08/07/2013
Avesthagen Limited, Bangalore, Karnataka India
Industry: Biopharma and Biotechnology
Worked as Scientist in a Breast cancer research project and involved in biopharma & bionutrition business development and Laboratory Operations.
Responsibilities •Proposed two scientific methods for identifying circulating low molecular-weight metabolites present in the blood plasma (whole genome sequencing project). •Etablished good laboratory set-up and maintaining the day-to-day research lab operations. Business Development for Bio-pharma and other Bio-nutrition products Responsibilities •Strategically planned & developed business model for Bio-nutra market. Assessed profitable market for company products and evaluated investor’s business model and finalized deals. •Identified and analyzed potential industries based on their products. Contacted probable companies and introduced our company products with broachers and product promotions. •Prepared CDAs, with prospective investors to generate business and revenue to the company.--
Researcher 04/01/2008 - 01/30/2011
Conway Institute of Biomolecular and Biomedical Institute, Dublin, Ireland Ireland
Industry: Biomedical
NEURODEGENERATIVE DISEASE (Functional & pathogenic amyloid) RESEARCH in Conway Institute of Bio molecular & Biomedical Research, UCD, Ireland.
Responsibilities • Established ex-vivo and in-vitro E. coli curli amyloid protein by protein-engineering methods. • Characterized WT Recombinant and Purified amyloid curli fibers using AFM, TEM and CLSM. • Performed qualitative and quantitative analysis of amyloid fibers using force-spectroscopy.--
Researcher 02/01/2006 - 01/30/2008
Umea University, Umea, Vaster Botten Sweden
Industry: Uiomolecular
NEURODEGENERATIVE DISEASE RESEARCH at UCMP, Umeå University, Sweden.
• Production, Purification and Acid-mediated aggregation of Amyloid fibrils. • Amyloid morphological study using AFM & its epitope detection by Western blot & MALDI-TOF. • Amyloid fibril stability evaluation study by histochemical dye Thioflavin-T.--

umea university 02/09/2005 - 08/24/2007
umea, vaster botten, Sweden
Degree: Master's Degree
Major:molecular biology
Minor:proteomics
2005-2007 Master of Science in Molecular biology, Umeå University, Sweden.