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Joao Banha Oliveira

Berlin Berlin, Germany

Phone: xxx-xxx-xxxx

Email: xxx@xxxx.xxx



  • Looking For: Senior Scientist, Project Manager

  • Occupation: Life, Physical, and Social science

  • Degree: Doctoral Degree

  • Career Level: Experienced

  • Languages: English, Portuguese, German, Spanish

Career Information:

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Highlights:Key Achievements: ? Advanced proprietary technology for drug-target identification resulting in its application to several commercial projects and adding value to the company. ? Created new analysis methods for validation, interpretation and presentation of complex mass-spectrometry data My achievements also allowed me to obtain a Ph.D. in Biochemistry.

Skills:biomarker, mass spectrometry, biochemistry, chemical biology, drug development, industry experience, immunology, flow cytometry, analytical thinking

Goal:I am looking forward to contribute to Biomarker Research associated with Drug Discovery and Development as well as improving Global Human Health in more ways than just research.

Honor:2010 -2013: Marie Curie Fellow, Early Stage Researcher, European Commission FP7 2008: Grant from the Luso-American Development foundation for internship in the U.S.A. 2004 -2010: Research fellowship from the National Institute of Health Dr. Ricardo Jorge 2003: PRODEP stipend from the University of Lisbon


Experiences:

Biochemistry Scientist 12/2010 - 09/2014
Caprotec Bioanalytics GmbH, Berlin, Berlin Germany
Industry: Pharmaceutical
caprotec bioanalytics GmbH is a Berlin-based biotechnology company that commercializes its proprietary technology, the Capture Compound Mass Spectrometry (CCMS) to identify the molecular targets of bioactive small molecules in collaboration with the pharmaceutical and the agrochemical industry.
Key Achievements: ? Advanced in-house proprietary technology to allow identification of drug targets in living cells ? Created new experimental assays and analysis methods for validation and interpretation of results ? Trained scientists and technicians in cellular culture, biochemistry and molecular biology methods ? Prepared and presented progress reports and elevator pitches to management in general meetings ? Co-organized meeting for international consortium with lectures and monitored practical courses ? Co-authored publication in peer-reviewed journal Technical Skills: ? Chemical Biology – identification of small-molecule targets in living cells or lysate ? Proteomics – protein analysis by mass spectrometry (Thermo Scientific LTQ-Orbitrap Velos) ? General Biochemistry methods – western/dot blotting, SDS-PAGE ? Molecular Biology – RNA extraction, PCR, semi-quantitative RT-PCR ? Cell Culture – mammalian cell lines, primary cells, isolation/culture of human PBMC IT skills: ? ImageJ for image/gel analysis and quantification ? Cytoscape for large data networks presentation and analysis ? MaxQuant software for MS data analysis, Statistica and SPSS for statistical analysis ? Learning R for data analysis and presentation--
Invited Research Scholar 08/2008 - 11/2008
Magee-Womens Research Institute, Pittsburgh, PA United States
Industry: Healthcare
Magee-Womens Research Institute is the largest independent research institute in the world focused solely on women’s health research. Its research scientists are engaged in health research that spans reproductive biology and development and diverse aspects of women’s health.
Guest at the Cancer Biomarkers Facility and Mass Spectrometry Platform. ? Researched serum biomarkers for rare auto-inflammatory disease to develop diagnostic tool Key Achievement: ? Acquired international experience in scientific and multi-cultural networking Technical Skills: ? Serum protein analysis preparation (Agilent MARS-14 and native gel electrophoresis) ? Peptide analysis by mass spectrometry (Thermo Scientific LTQ-XL/Orbitrap)--
Research Fellow 01/2005 - 12/2010
National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal
Industry: Healthcare and Diagnostics
The Portuguese National Health Institute Doutor Ricardo Jorge (INSA) is a public organization of the Ministry of Health, endowed with scientific, technical, administrative, financial and property of its own. All operative units composing the departments develop multidisciplinary programmes in problem-areas of Public Health, namely performing R&D, health monitoring, training, laboratory external quality assessment and general health services.
Founding member of the Cellular and Molecular Immunology Group and collaborator in the Proteomics Laboratory. ? Investigated the relationship of copper and iron metabolisms with the human immune system ? Researched serum biomarkers for rare auto-inflammatory disease to develop diagnostic tool ? Planned and participated in the elaboration of the yearly budget for the laboratory ? Supervised students and technicians in Flow Cytometry, cell culture and biochemistry methods Key Achievement: ? Co-authored 5 articles in peer-reviewed journals and gave 1 talk at International Science Conference (3rd European Proteomics Congress, Stockholm) Technical Skills: ? Flow Cytometry/FACS (BD Biosciences FACSCalibur) ? MALDI-TOF/TOF (Applied Biosystems 4700 Proteomics Analyzer) ? Western / Dot-Blotting ? SDS/2D-PAGE ? Protein radioactive labeling / Pulse-Chase ? Cell culture of immortalised cell lines and isolation/culture of human peripheral blood leukocytes ? Immunofluorescence confocal microscopy ? ELISA IT Skills: ? Advanced user of the Office™ suite (including some VBA programming in Excel) ? GraphPad PRISM, Statistica and SPSS ? CellQuest and FlowJo ? Proficient user of Windows and Mac environment ? Progenesis SameSpots (Nonlinear Dynamics)--

Education:

University of Lisbon 09/1999 - 12/2004
Lisbon, , Portugal
Degree: Master's Degree
Major:Biology
Research project focused on cellular and molecular immunology


University of Beira Interior 09/2005 - 12/2010
Covilhã, , Portugal
Degree: Master's Degree
Major:Clinical Immunology
Research project focused on Biomarker Discovery for a rare auto-inflammatory disease
Free University of Berlin 04/2011 - 04/2016
Berlin, Berlin, Germany
Degree: Doctoral Degree
Major:Biochemistry
Chemical Proteomics research for identification of drug targets in living cells

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