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| | Career Profile| Degree: | Master's Degree | | Career Level: | Qualified | | Occupation: | Life, Physical, and Social science | | Career Title: | Biological Technicians, Biological Scientists, All Other, Biochemists and Biophysicists | | Target Title: | research associate, research scientist | | Target Locations: | cambridge, MA , new york, NY , los angeles, CA | | Skills: | teamwork;research;adaptation;learning capacity; | | |
| Inaki Villanueva is research associate, research scientist(Life, Physical, and Social science) from Cambridge,MA United States. Experience
Massachusetts Institute of Technology - Knouse Laboratory Cambridge, MA United States
Industry: Academia
01/2022 - 08/2022
Visiting Scientist (6 month fellowship)
"Development of genome-wide CRISPR screening in the mammalian heart to study cardiac regeneration"
Mouse experimentation: in vivo and ex vivo work, surgical procedures, injection, transgenic colony
management (genotyping, mating, database recording), DNA isolation, primer design, PCR optimization.
Tissue processing: harvesting and processing, cryopreservation, immunostaining, confocal imaging an analysis, nuclei isolation, FACS.
Cell culture: Plate and suspension mammalian cell cultures (HEK-293, AML-12, HL-1) and bacterial cell
cultures.
Lentivirus-related techniques: CRISPR-Cas9 sgRNA plasmid preparation, production and purification.
Transfection, transduction and titration (flow cytometry), purification, western-blotting, injection, cell culture care and stock management.
Basque Government Bilbao, Basque Country Spain
06/2020 - 01/2022
Non-teaching Staff
Maintenance, disinfection and cleaning services.
Basque Center for Transfusion & Human Tissues. Research Unit in Cell Therapy & Steam Cells Bilbao, Basque Country Spain
Industry: Academia
07/2015 - 08/2015
Summer research student
"In vitro differentiation of stem cells to NK cells and subsequent characterization by flow cytometry"
Umbilical and peripheral blood processing, Mesenchymal Stem Cell isolation, Induced Pluripotent Stem Cell culture and differentiation, immunostaining, FC, characterization, data display and analysis.
Basque Center for Transfusion & Human Tissues. Research Unit in Cell Therapy & Steam Cells Bilbao, Basque Country Spain
Industry: Academia
07/2014 - 09/2014
Summer research student
"Isolation, culture and characterization of umbilical cord stem cells"
Blood sample processing, Peripheral Blood Mononuclear Cell isolation, immunostaining, FC, characterization, data display and analysis, cell culture techniques and stock maintenance.
Education
University of the Basque Country Bilbao, Basque Country, Spain
09/2014 - 02/2020
Degree: Bachelor's Degree
Major:Biotechnology
The Degree in Biotechnology results, fundamentally, from the integration of Molecular Biosciences with Engineering. It provides with the knowledge for the design and analysis of bioprocesses aimed at obtaining products, goods and services, as well as their management and control. These activities include bioindustries and the biomedical and pharmaceutical sectors: new diagnostic methods, vaccines, nanobiotechnology, new cancer therapies, etc. Its practical application transcends the health field to the veterinary, agricultural and food, chemical, environment and mining.
University of the Basque Country Bilbao, Basque Country, Spain
09/2017 - 06/2020
Degree: Bachelor's Degree
Major:Biochemistry and Molecular Biology
The Degree in Biochemistry and Molecular Biology develops the ability to understand, generate and transmit knowledge about biological processes at the molecular level in order to apply this knowledge to research and other fields.
European University Madrid, Madrid, Spain
09/2020 - 09/2021
Degree: Master's Degree
Major:Teacher Training
The Master's Degree in Teaching, is an official qualifying degree that allows the graduate to become a teacher of Secondary Education, Baccalaureate, Higher Level Training Cycles and in Official Language Schools. Specialization: Biology.
Valencian International University Valencia, Valencia, Spain
09/2021 - 10/2022
Degree: Master's Degree
Major:Biomedical Engineering
Biomedical engineering is primarily defined as the discipline that applies electrical, mechanical, and chemical principles, among others, to understand, modify, or control biological systems, and to design and manufacture products capable of monitoring physiological functions and assisting in diagnosis and treatment. from the patients.
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